Кузнецова Ольга Матвеевна


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 Lecture Course and Laboratory Manuals

in Biochemistry

2nd course of medical faculty

(First Semester: 2016/2017 educational year)




Laboratory works

1st week




2nd week


Subject, goals and main trends in Biological chemistry. Chemical components of living organism. Amino-Acids - as components of protein molecules. Structural and functional diversity of proteins. Physical-chemical properties of protein. Domen structure of proteins. Notion of shaperons. Methods for classification of protein. Specificity of structure of connective tissue proteins

Introduction talk.

Safety Instructions in a laboratory

Control test: Background knowledge of organic chemistry.

3rd week



Color reactions used for identification of proteins and amino acids.

Protein precipitation reactions

Dialysis of protein. Paper chromatography of amino acids.

4th week


Chemistry of conjugated (complex) proteins. Specificity of structure and biological activity of different kinds of complex proteins. Chemistry of Nucleic Acids. Chemical structure and biological role

Quantitative determination of protein. Construction of calibration curves.

Seminar: “Proteins and nucleic acids”

5th week



Effect of amylase on starch. Influence of temperature on amylase activity. Determination of alkaline phosphatase activity

6th week


Principles of catalysis: enzymes and ribozymes. Notion on activation energy and transition state of chemical reactions. Enzyme-substrate complexes. Dependence of the rate of enzymatic reactions from pH, temperature and substrate and enzyme concentration. Classification and nomenclature of enzymes. Isoenzymes. Immobilized enzymes. Determination of enzyme activity. Synthesis and degradation of enzymes. Enzymes inhibitors.

Colloquium №1. “Amino acids and simple proteins, complex proteins. Nucleic acids and enzymes.”

7th week



Quantitative determination of vitamin C in potatoes, and vitamin P in tea

8th week


Coenzymes - derivatives of vitamins. Functional role of coenzymes. Causes of hypovitaminosis states Chemistry of lipids. Classification and biological functions. Construction and functions of biomembranes

Seminar; Title: “Vitamins and lipids. Spectrophotometric determination of NADH and calculation of purity of commercial preparation

9th week



Seminar; Title: “Vitamins and lipids.

10th week


Hormones - the coordinators of biochemical processes. Coordination of Endocrine tissues. Chemical structure of hormones. Hormone-receptor interactions. Intracellular messengers. Molecular mechanisms of signal transduction. Eicosanoids: prostaglandins, prostaciclins, leucotriens, thromboxanes: biosynthesis, metabolism, biological effects. Inhibitors of prostaglandin and thromboxane synthesis. Notion on cytokines and formation of molecular immunology

Effect of hormones on glucose concentration in blood. Seminar: “Hormones”

11th week



Colloquium №2. “Vitamins, hormones, lipids”

12th week


Introduction to metabolism in living organism. Notion on metabolism and main metabolic pathways. Essential components of nutrition. Individual features and disturbances of metabolism. Principles of bioenergetics processes. ATP - as universal donator of free energy. The pathways of ATP synthesis: substrate and oxidative phosphorylation. Biological oxidation. Transport of electrons and protons across respiratory chain in inner mitochondrial membranes. Chemiosmotic theory of Mitchell.

Seminar: Chemistry of carbohydrates. Biological role of carbohydrates.

13th week



Specificity of amylase and sucrose action. Seminar; title: “The carbohydrate role in metabolism; accumulation of energy”

14th week


Carbohydrate metabolism. The possible pathways of glucose phosphate degradation. Anaerobic oxidation of glucose and glycogen, their regulation and energetic yield. Specificity of carbohydrate metabolism in muscle and liver. Cori cycle. Gluconeogenesis. Aerobic metabolism of carbohydrates. Pasteur effect. Oxidative decarboxylation of pyruvate. Krebs cycle (citric acid cycle – CAC) and its connection with biological oxidation. Regulation of glucose aerobic oxidation and its energetic yield.

Quantitative determination of glucose in blood. Construction of sugar curves

15th week



Quantitative determination of pyruvate in urine.

16th week


Pentose phosphate pathways of glucose oxidation in different tissues. Hormonal regulation of glycogen degradation and synthesis. The diseases of glycogen accumulation. Disturbances of carbohydrate metabolism. Diabetes mellitus. Biological role of substrates cycles.

Colloquium № 3. “Carbohydrate metabolism and its regulation”

17th week

18th week

19-24, 26-31/12

. Self-learning

Make-up work (After an extended illness, make-up work can be arranged with the teacher

Examination (Computer test)

Self-learning (Independent work) of student learning with a particular discipline can be done in the library of the university, classrooms, computer classrooms (laboratories) as well as at home.


2016-08-31 14:13:28
Congratulations on the new
educational year 2016-2017!

2016-04-12 09:33:58
Отработка 12.04 переносится на
14.04 (15.35)

2015-09-01 22:11:00
Сongratulations on the new
educational year!

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